What is USP plate count in HPLC?

What is USP plate count in HPLC?

Is the a guideline on plate count for system suitability. If I have a plate count of the first analyte of 1700, is the system suitable. 2000 is a recommendation, not a requirement.

What does number of theoretical plates mean?

Theoretical plate number (N) is an index that indicates column efficiency. It describes the number of plates as defined according to plate theory, and can be used to determine column efficiency based on calculation in which the larger the theoretical plate number the sharper the peaks.

How is USP tailing factor calculated?

The tailing factor is determined by drawing a perpendicular line from the peak centre to the baseline of the peak. Then the peak width and the front half-width are measured for the peak at 5% of the height of the peak. The tailing factor is simply the entire peak width divided by twice the front half-width.

What is a good theoretical plate count?

Generally, it ranges between 8000-12000 but it also depends upon the flow-rate, viscosity of mobile phase and molecular of the compound to be analyzed.

What is meant by theoretical plate?

A theoretical plate in many separation processes is a hypothetical zone or stage in which two phases, such as the liquid and vapor phases of a substance, establish an equilibrium with each other. Such equilibrium stages may also be referred to as an equilibrium stage, ideal stage, or a theoretical tray.

What’s the average plate count in the US?

Using the column mentioned above I am seeing plate counts values around 70,000 using USP calculations and 135,000 using EP calculations for the same peak in the same sample. Has anyone any input on this? Thanks

What is the theoretical plate count for ACQUITY UPLC?

Any input is appreciated. I am using an Acquity UPLC BEH 1.7µ, phenyl 2.1 x 150mm column and I am seeing theoretical plate counts (USP) around 70,000. I am more familiar with running HPLC methods and seeing theoretical plate counts up around 2,000.

How to calculate the accuracy of a plate count?

The United States Department of Agriculture (USDA) recommends using a grid to segment the counting area, then determining the average CFU/grid and multiplying this average CFU/grid by the number of grids on the plate (9). It is not clear to the author how either of these methods is greatly superior to guessing.

What do you need to know about USP 61?

The USP <61> test is a full quantitative analysis of a product to determine the Total Aerobic Microbial Count (TAMC) and Total Yeast and Mold Count (TYMC) present in the sample. It also includes a neutralization and recovery validation using specific microorganisms.

https://www.youtube.com/watch?v=yRAlDL67aSA