Table of Contents
- 1 What are the disadvantages of pour plate technique over spread plate technique?
- 2 What advantage does the spread plate method have over the streak plate method?
- 3 What is the difference between streak plate and pour plate?
- 4 What is the difference between streak plate method and spread plate technique?
- 5 What is the most important difference between a pour plate and spread plating?
- 6 What is the principle of pour plate technique?
- 7 How big of a volume can be spread on a spread plate?
- 8 How are cells deposited in a spread plate?
What are the disadvantages of pour plate technique over spread plate technique?
Disadvantages of Pour plate method
- Preparation for the pour plate method is time-consuming compared with the streak plate/and or spread plate technique.
- Loss of viability of heat-sensitive organisms coming into contact with hot agar.
- Embedded colonies are much smaller than those which happen to be on the surface.
What advantage does the spread plate method have over the streak plate method?
Heat sensitive microbes are not affected. No subsurface colonies appear in spread plate so isolation of the organism is easy.
What is the disadvantage of the streak plate technique?
Streak plating is a microbiology laboratory method that has two major disadvantages. Firstly, users will not be able to grow obligate anaerobes using this method. Secondly, only organisms that were viable in the original sample are able to be grown.
Why is spread plate better than pour plate?
With regard to the accuracy of these two techniques, pour plate has a higher accuracy than the spread plate. Moreover, unlike in a pour plate, a glass spreader is used to spread the sample evenly on the surface on a spread plate.
What is the difference between streak plate and pour plate?
Streak plate refers to a rapid qualitative isolation method for obtaining discrete colonies from a mixed population while pour plate refers to the method of choice for counting the number of colony-forming bacteria present in a liquid specimen.
What is the difference between streak plate method and spread plate technique?
The key difference between streak plate and spread plate is that the streak plate is used to isolate and purify a particular bacterial species from a mixture of bacteria while the spread plate is used to enumerate and quantify bacteria in a sample.
What is the difference between a streak plate and a pour plate?
Which is the most common isolation technique?
STREAK PLATE METHOD OF ISOLATION. The most common way of separating bacterial cells on the agar surface to obtain isolated colonies is the streak plate method we used in Lab 2 to inoculate a petri plate.
What is the most important difference between a pour plate and spread plating?
The main difference between pour plate and spread plate is that the molten agar is poured on to the inoculum during the preparation of the pour plate whereas inoculum is spread on the surface of the solidified agar during the preparation of the spread plate.
What is the principle of pour plate technique?
In a pour plate, a small amount of inoculum from a broth culture is added by pipette to the centre of a Petri dish. Cooled, but still molten, agar medium in a test tube or bottle is then poured into the Petri dish.
What are the advantages of the spread plate method?
There a few advantages of using the spread plate method. One of the advantages of using the spread plate method is it is better for isolating the bacteria colonies. It also is helpful in isolating organisms. Read More Compare the pour plate and streak plate methods of isolating bacteria?
Why are spread plates used for bacterial isolation?
1 Spread plate method is useful for isolation of aerobic microorganisms. 2 Bacterial culture is not exposed to high temperature as compare to pour plate method hence heat sensitive microbes are not affected. 3 In spread plate colonies appear only on surface of media, no subsurface colonies appear so isolation of the organism is easy.
How big of a volume can be spread on a spread plate?
Volume no greater than 0.1 ml can be spread on the nutrient agar plate because it would not soak well and may result in colonies to coalesce as they form. http://www.expertsmind.com/questions/explain-advantages-and-disadvantages-of-spread-plate-method-30175983.aspx
How are cells deposited in a spread plate?
The principle behind this method is that when the Media plate is spun, at some stage, single cells will be deposited with the bent glass rod (Spreader) onto the surface of the Agar media.
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