Table of Contents
- 1 What is the most likely reason for the poor appearance of the bacteria on this slide quizlet?
- 2 Which step is most likely to cause poor results in the Gram stain?
- 3 What are the three most important factors that determine the quality and clarity of a microscope image?
- 4 What are the common errors in staining methods?
- 5 What are some common sources of Gram staining errors?
- 6 What potential issues or problems does non compliance pose in the area of public health?
- 7 What should the magnification be on a bacteria slide?
- 8 Why are columnar cells taller than pseudostratified cells?
What is the most likely reason for the poor appearance of the bacteria on this slide quizlet?
Air drying of smeared bacterial cells. What is the most likely reason for the poor appearance of the bacteria on this slide? The slide was heat fixed for too long.
What would be some of the consequences for not leaving the stain on the slide long enough?
What are some consequences of not leaving a stain a smear long enough (under-staining)? Some consequences of under-staining are that the cells may lose their stain when washed with water or alcohol. This would make it difficult to identify the cell under the microscope.
Which step is most likely to cause poor results in the Gram stain?
the decolorization step
Excessive Decolorization It is clear that the decolorization step is the one most likely to cause problems in the Gram stain.
What would you observe if you Decolorized your slide too much?
What would you observe if you decolorized your slide too much? How would your cells appear? All of your cells would appear pink if you decolorized your cells too much with acetone-alcohol. Acetone-alcohol, if used too liberally, will wash the crystal violet out of the cells in your smear.
What are the three most important factors that determine the quality and clarity of a microscope image?
The primary factor in determining resolution is the objective numerical aperture, but resolution is also dependent upon the type of specimen, coherence of illumination, degree of aberration correction, and other factors such as contrast-enhancing methodology either in the optical system of the microscope or in the …
What does negative staining help to increase?
Negative staining, the embedding of a specimen in a layer of dried heavy metal solution, was introduced early on as a quick and easy specimen preparation technique that significantly increases the specimen contrast.
What are the common errors in staining methods?
The rank of errors is:
- Over decolourisation.
- Mixed cultures.
- Misread stains.
- Aged subcultures.
- Disorganisation.
- Inadequate fixation and Insufficient culture.
What are the consequences of over staining?
(Overstaining the bacterial smear may cause the cell wall disruption or totally destroy the cell wall which results in the loss of true morphological characteristics of the bacterial cell) 2.
What are some common sources of Gram staining errors?
What are the factors affecting the result of Gram staining?
The many variables that can affect this stain are age of the culture, amount of decolorizer used, the time of decolorization, the type of organism (acid-fast bacteria and spores do not stain well), thickness of the smear, and the general care of the stainer.
What potential issues or problems does non compliance pose in the area of public health?
Noncompliance has two important implications for public health: first, the patient remains infected and contagious and therefore can continue to spread the disease and second, by using chemotherapeutic agents for short periods of time, but not for the full course of treatment, the patient is selecting for the more drug …
How would you ensure that smears do not come off slides?
Place your carefully prepared fixed smear slides on the stain rack over the sink.
- Do one slide at a time.
- Cover the smear with any of the basic dyes available to you.
- You only need enough dye to cover the smear. The stain should not drip off the slide.
What should the magnification be on a bacteria slide?
The slide has three separate areas for each of three bacterial shapes (round, rod, and spiral). Select any one of the areas. Increase the magnification to 400X (known as “high dry” because greater magnification requires oil immersion).
Which is a false statement about the cytoskeleton?
Which of the following statements about the cytoskeleton is false? A. Once laid down, the elements of the cytoskeleton are fixed and remain permanently in place B. The cytoskeleton plays an important role in amoeboid motion C. The cytoskeleton is composed of three types of fibers: Microflaments, microtubules, and intermediate flaments D.
Why are columnar cells taller than pseudostratified cells?
Columnar cells are taller than they are _______. Pseudostratified cells look like more than one layer because of the arrangements of the cells’ nuclei. The general term for glands that secrete their products directly into ducts is ________.
What is enhanced when a stain is used to make a sample?
What is enhanced when a stain is used to make a sample, or parts of a sample, darker than the background in a slide? The ability to distinguish fine detail or discriminate between two very closely spaced objects is called ____. Microscopes make microorganisms appear larger than they are.