Menu Close

When would increasing the enzyme concentration have no effect on the reaction rate?

When would increasing the enzyme concentration have no effect on the reaction rate?

Terms in this set (25) What could cause no reaction in enzyme concentration lab? Based on your results from the enzyme concentration lab, when would increasing the enzyme concentration have no effect on the reaction rate? If the majority of the substrate has been consumed in the reaction.

What would happen if you increase the enzyme concentration?

By increasing the enzyme concentration, the maximum reaction rate greatly increases. Conclusions: The rate of a chemical reaction increases as the substrate concentration increases. Enzymes can greatly speed up the rate of a reaction.

Will decreasing the concentration of the enzyme increase the reaction rate?

One Enzyme, One Reaction If all the enzymes in a system are bound to substrates, additional substrate molecules must wait for an enzyme to become available following the completion of a reaction. This means that the rate of reactions will decrease as enzyme concentration decreases.

How does increasing substrate concentration affect reaction rate?

Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. As the enzyme molecules become saturated with substrate, this increase in reaction rate levels off. The rate of an enzyme-catalyzed reaction increases with an increase in the concentration of an enzyme.

What are the 4 factors that affect enzyme activity?

Several factors affect the rate at which enzymatic reactions proceed – temperature, pH, enzyme concentration, substrate concentration, and the presence of any inhibitors or activators.

Why does higher enzyme concentration increased rate of reaction?

Increasing Substrate Concentration increases the rate of reaction. This is because more substrate molecules will be colliding with enzyme molecules, so more product will be formed.

Can enzymes affect pH?

The effect of pH Enzymes are also sensitive to pH . Changing the pH of its surroundings will also change the shape of the active site of an enzyme. Many amino acids in an enzyme molecule carry a charge .

What happens when substrate concentration decreases?

The enzyme-bound molecule is called a substrate. Typically, an enzyme is combined with a substrate to reduce the activation energy of a chemical reaction. This means that as the enzyme concentration decreases, the reaction rate will decrease.

What happens if the enzyme concentration decreases?

If all of the enzymes in the system bind to the substrate, the additional substrate molecules must wait for the enzyme to become available after the reaction is complete. This means that as the enzyme concentration decreases, the reaction rate will decrease.

Why does rate of reaction increase with enzyme concentration?

What is the relationship between substrate concentration and the reaction rate?

As the substrate concentration increases the reaction rate does the same, because there is more substrate for the enzyme to react with. This is in fact the linear relationship. As the enzymes become more saturated the reaction levels off.

What is the problem in determining rates at low substrate concentration?

For low substrate concentrations (relative to the Km), depletion of the substrate causes the reaction to slow down more than at higher substrate concentration, so a low enzyme concentration is needed to maintain the initial rate long enough for the initial rate measurement to be made.

What happens when the concentration of an enzyme increases?

Initially, an increase in substrate concentration leads to an increase in the rate of an enzyme-catalyzed reaction. As the enzyme molecules become saturated with substrate, this increase in reaction rate levels off. The rate of an enzyme-catalyzed reaction increases with an increase in the concentration of an enzyme.

How is the rate of a reaction determined in the absence of enzymes?

Variables: Methods: In the absence of enzymes, the rate of a reaction can be thought to increase linearly with substrate concentration. The reaction rate is given as dp/dt, or the change in product over time: where S is the substrate concentration, and k is the frequency at which substrate is converted to product.

How does substrate concentration affect the reaction rate?

Increasing substrate concentration only affects the reaction rate in so far is it affects the proportion of enzyme molecules involved in enzyme–substrate complex, which reaches a maximum when all of the enzyme is in such complexes. The question presents a rate constant for only one of the reactions involved in an enzyme-catalysed reaction.

When does the rate of a chemical reaction increase?

Conclusions: The rate of a chemical reaction increases as the substrate concentration increases. Enzymes can greatly speed up the rate of a reaction. However, enzymes become saturated when the substrate concentration is high. Additionally, the reaction rate depends on properties of the enzyme (K, kcat) and the enzyme concentration (E).